Journal: PLOS Neglected Tropical Diseases

Article Title: Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates

doi: 10.1371/journal.pntd.0011016

Figure Lengend Snippet: A and B. Surface expression or secretion of galectins. CFSE-labeled TH17 trophozoites (green) were co-incubated with h VECs (moi 1:3) were sampled at intervals for the detection of galectin-1 (red) and galectin-3 (cyan) by IFA (A), while these galectins in the conditioned medium were detected by ELISA (B). PI, post-infection. Adherent trophozoites in A are indicated by white triangles. C, D, and E. T1 or TH17 trophozoites were incubated in fresh or conditioned KSFM (D) from h VECs cultures for 30 min. Cells were fixed for detection of galectin-1 or -3 by IFA without permeation for (C) or with permeation for (D and E) before immune detection. E. IFA co-staining with anti-galectin-3 antibody and LysoTracker. F and G. T1 or TH17 trophozoites were incubated with FITC-conjugated recombinant galectin-1 or galectin-3 and analyzed by flow cytometry (F) or fluorescence microscopy (G). The bar graph is shown by mean ± SD. All assays were repeated three times and the representative data are shown here. Differences were statistically analyzed by Student’s t-test, with P <0.01(**) and P <0.05(*).

Article Snippet: Immortalized human vaginal epithelial cells ( h VECs, VK2/E6E7, ATCC CRL-2616) and HeLa cells were respectively maintained in semi-defined keratinocyte serum-free medium (KSFM) (GIBCO-BRL 17005–042, ThermoFisher Scientific) and DMEM high glucose medium (12100046, ThermoFisher Scientific) with 10% fetal bovine serum at 37°C and 5% CO 2 .

Techniques: Expressing, Labeling, Incubation, Enzyme-linked Immunosorbent Assay, Infection, Staining, Recombinant, Flow Cytometry, Fluorescence, Microscopy

Journal: PLOS Neglected Tropical Diseases

Article Title: Distinct features of the host-parasite interactions between nonadherent and adherent Trichomonas vaginalis isolates

doi: 10.1371/journal.pntd.0011016

Figure Lengend Snippet: The tract of a single TH17 or T1 trophozoite migrating on a glass slide for 1 min is depicted in A. The migratory paths from ~50 trophozoites are shown in B, with the average track displacement length (TDL). The average velocities with standard deviations were statistically analyzed by Student’s t-tests as shown in C. The relative velocity of T1 in D. or TH17 in E., the trophozoites cultured in KSFM or with h VECs over 15 min was normalized by the average velocity of those in 1 min post-incubation in KSFM. The assays were repeated three times. For D. and E., the statistical analysis was measured by Bonferroni post hoc tests (n = 10 to 30 for each group). The error bars represent standard deviations, P <0.01(**) and P <0.05(*).

Article Snippet: Immortalized human vaginal epithelial cells ( h VECs, VK2/E6E7, ATCC CRL-2616) and HeLa cells were respectively maintained in semi-defined keratinocyte serum-free medium (KSFM) (GIBCO-BRL 17005–042, ThermoFisher Scientific) and DMEM high glucose medium (12100046, ThermoFisher Scientific) with 10% fetal bovine serum at 37°C and 5% CO 2 .

Techniques: Cell Culture, Incubation

Journal: Scientific Reports

Article Title: Production and properties of non-cytotoxic pyomelanin by laccase and comparison to bacterial and synthetic pigments

doi: 10.1038/s41598-021-87328-2

Figure Lengend Snippet: Effect of Pyo ENZ on scavenging ROS generated by UVA irradiation on keratinocyte cells.

Article Snippet: Human epidermal keratinocytes neonatal cells were maintained in a complete keratinocyte serum-free medium (Panserin 412, from DUTSCHER) supplemented with bovine pituitary extract (30 μg/mL), recombinant epidermic growth factor (rEGF, 0.2 ng/mL), and an antibiotic cocktail of 10 U/mL penicillin-100 μg/mL streptomycin.

Techniques: Generated, Irradiation, Fluorescence, Control